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Elimination of interferences in flow-injection amperometric determination of glucose in blood serum using immobilized glucose oxidase

✍ Scribed by Wojciech Matuszewski; Marek Trojanowicz; Andrzej Lewenstam

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
720 KB
Volume
2
Category
Article
ISSN
1040-0397

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✦ Synopsis

A flow-injection system with anodic aniperometric detection for glucose determination in human blood serum samples was optimized by a comparison of various arrangements of enzyme immobilization and the elimination o f interferences. The best results were obtained using a compact membrane biosensor in a wall-jet, flow-through detector with a platinum disk electrode covered with an evaporated Nafion layer, a polyester membrane with immobilized glucose oxidase, and another protective polyester mernbrane. In the optimized conditions, a linear response was observed up t o 25 mM glucose with a detection limit of 200 p M glucose and a sampling rate o f 120 samples/h for a 20 pL sample volume. Results of glucose deterniination in human serum were compared with a Heckman Glucose Analyzer 2 and a Kone Dynamic analyzer.

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