Amperometric flow-injection determination of glucose, urate and cholesterol and blood serum by using some immobilized enzyme reactors and a poly(1,2-diaminobenzene)-coated platinum electrode
✍ Scribed by Toshio Yao; Masahiro Satomura; Taketoshi Nakahara
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 525 KB
- Volume
- 296
- Category
- Article
- ISSN
- 0003-2670
No coin nor oath required. For personal study only.
✦ Synopsis
Glucose, uric acid and cholesterol in blood serum were assayed simultaneously in a flow-injection system by using a 16-way switching valve and a parallel configuration of three immobilized enzyme reactors. A polyO,2diaminobenzene)_coated platinum electrode was used to detect selectively hydrogen peroxide generated enzymatically into the enzyme reactors, without any interferences from oxidizable species and proteins present in serum. The precision (coefficient of variation) of the assay was better than 2% for the simultaneous assay of three species in human control serum. The assay speed was up to 38 samples per hour.