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Enzyme electrode for the successive detection of hypoxanthine and inosine

✍ Scribed by Toshio Yao


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
334 KB
Volume
281
Category
Article
ISSN
0003-2670

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✦ Synopsis


An enzyme electrode for the successive detection of hypoxanthine and inosine was constructed by co-immobilizing nucleoside phosphorylase and xanthine oxidase on a Nafion-coated platinum disc electrode. The successive responses for hypoxanthine and inosine were obtained in 0.1 M borate buffer (pH 7.5) without and with phosphate, respectively. The calibration graphs for hypoxanthine and inosine were linear up to 2 x 10e4 M. The sensor could be used repeatedly for at least 20 days without deterioration of the response.


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