A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase ( NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafionparaquat film. This sensor responds
Enzyme electrode for the successive detection of hypoxanthine and inosine
β Scribed by Toshio Yao
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 334 KB
- Volume
- 281
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
An enzyme electrode for the successive detection of hypoxanthine and inosine was constructed by co-immobilizing nucleoside phosphorylase and xanthine oxidase on a Nafion-coated platinum disc electrode. The successive responses for hypoxanthine and inosine were obtained in 0.1 M borate buffer (pH 7.5) without and with phosphate, respectively. The calibration graphs for hypoxanthine and inosine were linear up to 2 x 10e4 M. The sensor could be used repeatedly for at least 20 days without deterioration of the response.
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