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Amperometric sensor for hypoxanthine and xanthine based on the detection of uric acid

✍ Scribed by E. Gonzalez; F. Pariente; E. Lorenzo; L. Hernandez

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
562 KB
Volume
242
Category
Article
ISSN
0003-2670

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✦ Synopsis

The construction and response of an immobilized enzyme modified electrode as an amperometric sensor is described. Xanthine oxidase was adsorbed on a carbon paste electrode and physically entrapped with a semipermeable membrane.

Uric acid, the product of the enzymatic reaction, was oxidized electrochemically at +0.4 V vs. Ag/AgCl, yielding a steady-state current directly related to the bulk concentration of the substrate. Hypoxanthine and xanthine were determined in the range 5-100 pM at pH 7.2 with good precision. Interferences are discussed.

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