Enzyme Dynamics and Regulation

✍ Scribed by V. Mizrahi, P. A. Benkovic, R. D. Kuchta, M. C. Young, K. A. Johnson, S. J. Benkovic (auth.), P. Boon Chock, Charles Y. Huang, C. L. Tsou, Jerry H. Wang (eds.)

• Publisher: Springer-Verlag New York
• Year: 1988
• Tongue: English
• Leaves: 440
• Edition: 1
• Category: Library

✦ Synopsis

Recent developments in concepts and techniques have brought enzyme research to a changing yet exciting stage. Enzymes have served as indispensable tools in the phenomenal rise of molecular biology, and the resultant biotechnology thrusts enzymes to new heights and territories. This volume, the proceedings of a recent symposium on the Dynamics of Soluble and Immobilized Enzyme Systems, provides a current overview of the field to help scientists utilize long-established and newly acquired information.

✦ Table of Contents

Front Matter....Pages i-xxi
Mechanistic Studies on DNA Polymerase I....Pages 1-5
Assembly and Catalytic Functions of the Subunits of Succinyl Coenzyme A Synthetase....Pages 6-16
Fatty Acid Synthetase of Chicken Liver: A Novel Active-Site Structure for Condensation Comprised of SH Groups from a Cysteine Residue and an Oscillating Phosphopantetheine Swinging Arm on Adjacent Subunits....Pages 17-27
Regulatory Properties of Glucokinase....Pages 28-39
Mechanism of Activation of Calmodulin-Dependent Phosphatase by Divalent Metal Ions....Pages 40-47
Studies on the Mechanism and Molecular Mode of Regulation of Fructose-1,6-bisphosphatase....Pages 48-54
Phosphorylation and dAMP Inhibition of Snake Muscle Fructose-1,6-bisphosphatase....Pages 55-61
Identification and Characterization of Intermediates in the Mechanism of Enzyme Action....Pages 62-70
Conformational Dynamics in RNA—Protein Interactions: Immobilization of the Functional Domains in tRNA fMet and Methionyl-tRNA Synthetase....Pages 71-76
Structural and Kinetic Studies of Escherichia coli Glutamine Synthetase....Pages 77-83
Cu,Zn Superoxide Dismutase: A Case of Metalloenzyme Catalysis in Which the Protein Moiety Plays a Major Role....Pages 84-91
Dynamic Participation of Protein Domains in Catalysis by 2-Oxo Acid Dehydrogenase Multienzyme Complexes....Pages 92-101
Membrane-Bound GTP-Transducin Efficiently Activates Retinal cGMP Phosphodiesterase....Pages 102-112
Signal Transduction in the β -Adrenoceptor-Dependent Adenylate Cyclase....Pages 113-120
Acetyl-CoA Carboxylase: Correlation of Phosphorylation State with Allosteric Properties and Physiological State....Pages 121-127
Role of Cyclic Cascades in Metabolic Regulation....Pages 128-135
Regulation of Glutamine Synthetase Activity and Its Biosynthesis in Escherichia coli : Mediation by Three Cycles of Covalent Modification....Pages 136-145
Regulation of ATP, Mg-Dependent Protein Phosphatases....Pages 146-154
Molecular Mechanisms of Allosteric Regulation in Aspartate Transcarbamylase....Pages 155-161
Dynamic Interactions of the Second Messenger Systems....Pages 162-174
Role of Multienzyme Complexes in the Integration of Cellular Metabolism....Pages 175-180
Dynamics of Deoxynucleotide Synthesis in Relation to DNA Replication....Pages 181-189
Differential Stabilization of Left-Handed Z-DNA and Z-RNA In Vitro and In Vivo....Pages 190-199
Interaction of Restriction Endonucleases with Phosphorothioate DNA....Pages 200-205
Role of Conformational Dynamics of Myosin in Muscle Contraction....Pages 206-216
Enzymatic Modulation of Cytoskeletal Self-Assembly: ADP Ribosylation of Microtubule Protein Components....Pages 217-223
Human Angiogenin: An Organogenic Protein....Pages 224-225
Structure and Mechanism of Action of a Membrane-Bound Enzyme: Chloroplast Coupling Factor....Pages 226-234
Electrostatic Effects and the Dynamics of Multienzyme Reactions at the Surface of Plant Cells....Pages 235-246
Energy Transduction by Electroconformational Coupling....Pages 247-260
Topics in Petroleum Biotechnology....Pages 261-267
Experimental Determination of Rate Constants in Enzymatic Reactions....Pages 268-274
Applications of Alternative Substrate Kinetics: In Vivo and In Vitro Data on Aminoglycoside Antibiotic Inactivating Enzymes....Pages 275-288
Kinetics of Irreversible Modification of Enzyme Activity....Pages 289-300
Applications of Stable Isotopes in Biochemistry....Pages 301-311
Enzyme Dynamics in Nonaqueous Media at Subzero Temperatures....Pages 312-321
Kinetic—Structural Organization of Enzyme Systems....Pages 322-326
Relation Between Structure and Function in cAMP-Dependent Protein Kinases....Pages 327-341
Conformation and Dynamics of Oligomeric Enzymes....Pages 342-350
Coenzyme Binding Site of NADP-Dependent Isocitrate Dehydrogenase....Pages 351-361
Significance of Domain Structure of Calmodulin on the Activation of Ca 2+ —Calmodulin-Requiring Enzymes....Pages 362-366
Modification of Leucyl-tRNA Synthetase by Affinity Labeling and Limited Proteolysis....Pages 367-376
NAD Metabolism in Eukaryotic Cells: Purification and Characterization of NMN Adenylyltransferase from Baker’s Yeast....Pages 377-384
Specific β - d -Fucosidase from Aspergillus phoenicis ....Pages 385-393
Formation of Active Aspartate Transcarbamoylase from Defective Polypeptide Chains Produced by Site-Directed Mutagenesis....Pages 394-404
Catalytic Mechanisms Revealed by Protein Engineering of Tyrosyl-tRNA Synthetase....Pages 405-410
Site-Directed Mutagenesis of Alkaline Phosphatase....Pages 411-414
Back Matter....Pages 415-421

✦ Subjects

Biochemistry, general; Cell Biology; Biotechnology