Enzyme Kinetics and Mechanism Part D: Developments in Enzyme Dynamics

✍ Scribed by Daniel L. Purich (Eds.)

Publisher: Academic Press
Year: 1995
Tongue: English
Leaves: 680
Series: Methods in Enzymology 249
Edition: 1
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

General Description of the Volume:
This volume, as do the other Enzyme Kinetics and Mechanism volumes in the Methods in Enzymology series, provides treatment of dynamic and chemical approaches for investigating enzyme catalysis and regulation, as well as designing metabolic inhibitors. It will greatly interest those involved in enzyme chemistry, metabolic control, and drug design. It should also interest those developing commercial applications for enzymes whose properties have been re-engineered using recombinant DNA technology and site-directed mutagenesis.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features
Mechanisms of enzyme catalysis and inhibition
Enzyme structure and function
Regulatory control of enzymes
Action of catalytic antibodies and ribozymes

✦ Table of Contents

Content:
Contributors to volume 249
Pages vii-viii

Preface
Page ix
Daniel L. Purich

Volume in series
Pages xi-xxv

[1] Transient kinetic approaches to enzyme mechanisms Original Research Article
Pages 3-37
Carol A. Fierke, Gordon G. Hammes

[2] Rapid quench kinetic analysis of polymerases, adenosinetriphosphatases, and enzyme intermediates Original Research Article
Pages 38-61
Kenneth A. Johnson

[3] Analysis of enzyme progress curves by nonlinear regression Original Research Article
Pages 61-90
Ronald G. Duggleby

[4] Site-directed mutagenesis: A tool for studying enzyme catalysis Original Research Article
Pages 91-119
Bryce V. Plapp

[5] Reversible enzyme inhibitors as mechanistic probes Original Research Article
Pages 123-143
Herbert J. Fromm

[6] Kinetics of slow and tight-binding inhibitors Original Research Article
Pages 144-180
Stefan E. Szedlacsek, Ronald G. Duggleby

[7] Kinetic method for determination of dissociation constants nucleotide complexes Original Research Article
Pages 181-188
W.W. Cleland

[8] Product inhibition applications Original Research Article
Pages 188-211
Bruce F. Cooper, Frederick B. Rudolph

[9] Kinetics of iso mechanisms Original Research Article
Pages 211-240
Karen L. Rebholz, Dexter B. Northrop

[10] Mechanism-based enzyme inactivators Original Research Article
Pages 240-283
Richard B. Silverman

[11] Transition state and multisubstrate analog inhibitors Original Research Article
Pages 284-312
Anna Radzicka, Richard Wolfenden

[12] Partition analysis: Detecting enzyme reaction cycle intermediates Original Research Article
Pages 315-340
Irwin Arose

[13] Isotope effects: Determination of enzyme transition state structure Original Research Article
Pages 341-373
W.W. Cleland

[14] Hydrogen tunneling in enzyme catalysis Original Research Article
Pages 373-397
Brian J. Bahnson, Judith P. Klinman

[15] Positional isotope exchange as probe of enzyme action Original Research Article
Pages 398-425
Leisha S. Mullins, Frank M. Raushell

[16] Manipulating phosphorus stereospecificity of adenylate kinase by site-directed mutagenesis Original Research Article
Pages 425-443
Ming-Daw Tsai, Ru-Tai Jiang, Terri Dahnke, Zhengtao Shi

[17] Equilibrium isotope exchange in enzyme catalysis Original Research Article
Pages 443-479
Frederick C. Wedler

[18] Proton transfer in carbonic anhydrase measured by equilibrium isotope exchange Original Research Article
Pages 479-503
David N. Silverman

[19] Expression of properly folded catalytic antibodies in Escherichia coli Original Research Article
Pages 507-519
Jon D. Stewart, Irene Lee, Bruce A. Posner, Stephen J. Benkovic

[20] Cooperativity in enzyme function: Equilibrium and kinetic aspects Original Research Article
Pages 519-567
Kenneth E. Nest

[21] Kinetic basis for interfacial catalysis by phospholipase A₂ Original Research Article
Pages 567-614
Mahendra Kumar Jain, Michael H. Gelb, Joseph Rogers, Otto G. Berg

Author index
Pages 615-640

Subject index
Pages 641-662

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