Enzymatic pretreatments of rice bran, using commercial carbohydrases to improve nitrogen extractability, were investigated. The pretreatment used a response surface method which included the variations in concentration of Viscozyme L (0È120 FBG unit) and Celluclast 1.5L (0È360 NC unit), time of incu
Enzyme-assisted water-extraction of oil and protein from rice bran
✍ Scribed by P Hanmoungjai; D L Pyle; K Niranjan
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2002
- Tongue
- English
- Weight
- 228 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0268-2575
- DOI
- 10.1002/jctb.635
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✦ Synopsis
Abstract
Enzymatic water‐extraction of oil and proteins from rice bran was studied in a laboratory‐scale set‐up. The effects of the following enzymes – Celluclast 1.5L, hemicellulase, Pectinex Ultra SP‐L, Viscozyme L, Alcalase 0.6L and papain – on oil and protein extraction yields, and the level of reducing sugars in the extract were investigated. The results showed that Alcalase was most effective in enhancing oil and protein extraction yields. Papain was found to be superior to all carbohydrase enzymes but it gave lower yields than Alcalase. Celluclast 1.5L, hemicellulase, Pectinex Ultra SP‐L and Viscozyme L did not affect yields significantly but increased the level of reducing sugars in the extract.
© 2002 Society of Chemical Industry
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The extractability profile of rice bran proteins under different conditions were studied to determine optimal conditions of extraction. Milling and differential sieving of rice bran increased the protein content in fine fraction by 7%. Maximum extractability of protein from defatted rice bran at pH