Application of Carbohydrases in Extracting Protein from Rice Bran

✍ Scribed by Ansharullah, ; Hourigan, James A; Chesterman, Colin F

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 344 KB
• Volume: 74
• Category: Article
• ISSN: 0022-5142
• DOI: 10.1002/(sici)1097-0010(199706)74:2<141::aid-jsfa778>3.0.co;2-5

✦ Synopsis

Enzymatic pretreatments of rice bran, using commercial carbohydrases to improve nitrogen extractability, were investigated. The pretreatment used a response surface method which included the variations in concentration of Viscozyme L (0È120 FBG unit) and Celluclast 1.5L (0È360 NC unit), time of incubation (1È5 h) and pH (3É8È5É4). The experiments were conducted at two di †erent temperatures, 40 and 50¡C. At 40¡C, the maximum nitrogen extraction predicted by the model was obtained when the ViscozymeÈCelluclast mixture at pH 3É8 with incubation time of 5 h was applied, giving an extracted N of 51%. At the process temperature of 50¡C, the model predicted that the use of Viscozyme alone, under similar conditions, would have a most signiÐcant e †ect in increasing the efficacy of the nitrogen extraction, producing a 57% extracted N. This enzymatic pretreatment method improved the nitrogen extractability of rice bran at the neutral pH.