𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Enzymatic preparation of 3H-labeled β-ureidoisobutyric acid and β-aminoisobutyric acid from 3H-thymidine

✍ Scribed by Anthony F. Shields; Laura Kozell

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
164 KB
Volume
28
Category
Article
ISSN
0022-2135

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

We have synthesized β‐aminoisobutyric acid (3‐amino‐2‐[^3^H]methyl‐propanoic acid) and β‐ureidoisobutyric acid (3‐[(aminocarbonyl)amino]‐2‐[^3^H]methyl‐propanoic acid) starting with [^3^]thymidine (1‐(2‐deoxy‐β‐D‐ribo‐furanosyl)‐5‐[^3^H]methyluracil). We have developed a simplified enzymatic preparation using a dialyzed supernatant of homogenized dog liver. The homogenate was incubated with [^3^H]thymidine (41 Ci/mmol) in the presence of NADPH, and β‐ureidopropionic acid. After extraction the supernatant was purified by HPLC.

📜 SIMILAR VOLUMES

Preparation of 14C labeled β-aminoisobut
Preparation of 14C labeled β-aminoisobutyric acid (3 Amino-2 methyl-propionic acid, 314C)
✍ J. H. Lukas; G. B. Gerber 📂 Article 📅 1965 🏛 John Wiley and Sons 🌐 French ⚖ 81 KB

Preparation of 14C labeled p-aminoisobutyric acid (3 Amino-2 methyl-propionic acid, 314C) \* In the course of studies on the catabolism of thymidine the need emerged to study in greater detail its metabolite (l), p aminoisobutyric acid (BAIBA). This substance, labeled with 14C in the 3-C position,

Enzymic preparation of specifically 14C
Enzymic preparation of specifically 14C and 3H labelled shikimic acids
✍ K.-H. Scharf; M. H. Zenk 📂 Article 📅 1971 🏛 John Wiley and Sons 🌐 French ⚖ 344 KB

## D-Shikimic acids I-I4C, 6-14C and 7-(carboxyl) 14C have been biosyntheticaily prepared with specific activities of about 25 mCi/ mmole and with overall yields of 45% based on pyruvate 3,2 or I-1% as the respective starting materials. A crude cellfree homogenate of E. coli 83-24 was used to cat

Enzymatic synthesis of 5-3H-indole-3-ace
Enzymatic synthesis of 5-3H-indole-3-acetic acid and 5-3H-indole-3-acetyl-myo-inositol from 5-3H-L-tryptophan
✍ Lech Michalczuk; John R. Chisnell 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 French ⚖ 309 KB

## Abstract Labeled 1‐tryptophan is converted to indole‐3‐acetamide and then to indole‐3‐acetic acid by enzymes from __Pseudomonas savastanoi__. Labeled indole‐3‐acetic acid can be converted to indole‐3‐acetyl‐1‐__0__‐β‐D‐glucose and to indole‐3‐acetyl‐__myo__‐inositol by enzymes from kernels of __

The synthesis and resolution of tritiate
The synthesis and resolution of tritiated 4-amino-3-phenylbutanoic acid ([3H]R- and [3H]S-β-phenylgaba)
✍ Rujee K. Duke; Robin D. Allan; Colleen A. Drew; Graham A. R. Johnston; Kenneth N 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 French ⚖ 396 KB

## Abstract A synthesis of [^3^H]__R__‐ and [^3^H]__S__‐4‐amino‐3‐phenylbutanoic acid ([^3^H]__R__‐ and [^3^H]__S__‐β‐phenylGABA) from the unsaturated intermediate by catalytic hydrogenation using tritium gas in the presence of palladium on charcoal is reported. The [^3^H]__R__‐ and [^3^H]__S__‐dia