A method for the synthesis of deuterium-labelled tryptophan and its enzymatic conversion to indole-3-pyruvic acid ( ( Hs) IPyA) using amino acid oxidase is described. an internal standard for the quantitation of IPyA by mass spectrometry in plant extracts and for estimating the breakdown of IPyA to
Enzymatic synthesis of 5-3H-indole-3-acetic acid and 5-3H-indole-3-acetyl-myo-inositol from 5-3H-L-tryptophan
✍ Scribed by Lech Michalczuk; John R. Chisnell
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- French
- Weight
- 309 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0022-2135
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✦ Synopsis
Abstract
Labeled 1‐tryptophan is converted to indole‐3‐acetamide and then to indole‐3‐acetic acid by enzymes from Pseudomonas savastanoi. Labeled indole‐3‐acetic acid can be converted to indole‐3‐acetyl‐1‐0‐β‐D‐glucose and to indole‐3‐acetyl‐myo‐inositol by enzymes from kernels of Zea mays sweet corn.
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Synthesis of 5H-Pyrazino(2,3-b)indoles from Indole-2,3-dione Derivatives. -The reaction of the N-acetylisatins (I) with ethylenediamine yields the products (III). After dehydrogenation these compounds and their analogues (IV) after deacetylation are cyclized by heating to the title products (VI). N
## Abstract The synthesis of the title compounds 5__H__, 11__H__‐pyrido[2′,3′:2,3]thiopyrano[4,3‐__b__]indoles was accomplished by the Fischer indole cyclization of some 2,3‐dihydrothiopyrano[2,3‐__b__]pyridin‐4(4__H__)‐one phenylhydrazones and 7‐methyl‐2,3‐dihydrothiopyrano[2,3‐__b__]pyridin‐4(4__
## Abstract Syntheses of 5,7‐^3^H~2~ and 4,6‐^3^H~2~ tryptophan, respectively from 2,4,6‐^3^H~3~‐ and 3,5‐^3^H~2~‐aniline, were obtained by Fischer cyclization of suitable labelled phenylhydrazones.