Enzymatic microdetermination of serum free fatty acids
โ Scribed by Sakayu Shimizu; Keiko Inoue; Yoshiki Tani; Hideaki Yamada
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 350 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
A reliable, simple. and rapid enzymatic I Abbreviations used: FFA, free fatty acid; ACS, acyl-CoA synthetase.
described above. The results of actual assays for FFA in various serum samples are compared with those obtained by the published calorimetric analysis.
Methods
CoA was kindly given by Kyowa Hakko Kogyo Company, Tokyo, and Wakamoto
๐ SIMILAR VOLUMES
## Abstract An environmentally benign and volume efficient process for enzymatic production of alkanolamides is described. Immobilized __Candida antarctica__ lipase B, Novozymยฎ435, was used to catalyze the condensation of lauric acid with monoethanolamine. The reaction temperature of 90ยฐC was requi
A simple, highly specific, and sensitive bioluminescent method for determination of free fatty acids in unextracted plasma or serum has been developed. The method is based on the activation of free fatty acids by acyl-CoA synthetase (EC 6.2.1.3). The pyrophosphate formed is used to phosphorylate fru
The most frequently used method for estimating free fatty acids (FFA) in biological tissues is the titrimetric procedure of Dole (1, 2). This technique requires appreciable experience and observer bias can easily affect, the titration values (3). A sensitive calorimetric method was proposed by Dunc