A reliable, simple. and rapid enzymatic I Abbreviations used: FFA, free fatty acid; ACS, acyl-CoA synthetase. described above. The results of actual assays for FFA in various serum samples are compared with those obtained by the published calorimetric analysis. ## Methods CoA was kindly given by
Colorimetric microdetermination of plasma free fatty acids
โ Scribed by Donald C. Kvam; J. G. Schmidt; D. A. Riggilo; D. G. Gallo
- Publisher
- John Wiley and Sons
- Year
- 1964
- Tongue
- English
- Weight
- 228 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0022-3549
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The most frequently used method for estimating free fatty acids (FFA) in biological tissues is the titrimetric procedure of Dole (1, 2). This technique requires appreciable experience and observer bias can easily affect, the titration values (3). A sensitive calorimetric method was proposed by Dunc
A simple, highly specific, and sensitive bioluminescent method for determination of free fatty acids in unextracted plasma or serum has been developed. The method is based on the activation of free fatty acids by acyl-CoA synthetase (EC 6.2.1.3). The pyrophosphate formed is used to phosphorylate fru