Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts
β Scribed by Timothy P. Carr; Catharine J. Andresen; Lawrence L. Rudel
- Book ID
- 107740501
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 440 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0009-9120
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π SIMILAR VOLUMES
A procedure for the determination of free and total cholesterol in lipid extracts is described. The method for free cholesterol employs cholesterol oxidase to generate H202 and peroxidase to catalyze the reaction of Hz02 with o-dianisidine to yield a colored product. For the determination of total
The estimation of cholesterol in tissue lipid extracts is often complicated by the presence of substances which interfere with the calorimetric methods generally used. In these circumstances cholesterol is usually precipitated as the digitonide, and can then be estimated in the accustomed way. Separ