Estimation of cholesterol in tissue lipid extracts after separation by thin-layer chromatography

✍ Scribed by Lois M. Cherry

• Book ID: 104146907
• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 331 KB
• Volume: 39
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98040-9

✦ Synopsis

The estimation of cholesterol in tissue lipid extracts is often complicated by the presence of substances which interfere with the calorimetric methods generally used. In these circumstances cholesterol is usually precipitated as the digitonide, and can then be estimated in the accustomed way. Separation of the constituents of the extract by thin-layer chromatography offers an alternative method of isolation, but entails the modification of techniques for the subsequent recovery and estimation of cholesterol. This note is concerned with the adaptation of existing methods for routine use under these conditions. The requirements for satisfactory preliminary chromatographic separation are not examined, since these will obviously vary with the type of lipid extract and the preferences of individual workers.