One-dimensional thin-layer chromatographic separation of phospholipids and lysophospholipids from tissue lipid extracts
โ Scribed by Wen-Qi Wang; Anders Gustafson
- Publisher
- Elsevier Science
- Year
- 1992
- Weight
- 357 KB
- Volume
- 581
- Category
- Article
- ISSN
- 0378-4347
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๐ SIMILAR VOLUMES
A method for the quantitation of small amounts of phospholipids derived from biological sources is described. Total phospholipid is determined by mineralization followed by the estimation of liberated phosphate by means of malachite green. The main phospholipid species are separated by one-dimension
A simple method for separating citrate, malate, aspartate, and glutamate in crude extracts by thin-layer chromatography on plastic sheets coated with a strongly basic anion exchange resin has been developed. The method is useful for determining the radioactivity of these compounds in tracer experime