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Enhancing the Enantioselectivity of an Epoxide Hydrolase by Directed Evolution

✍ Scribed by Reetz, Manfred T.; Torre, Claudia; Eipper, Andreas; Lohmer, Renate; Hermes, Marcus; Brunner, Birgit; Maichele, Andrea; Bocola, Marco; Arand, Michael; Cronin, Annette; Genzel, Yvonne; Archelas, Alain; Furstoss, Roland

Book ID
127330219
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
103 KB
Volume
6
Category
Article
ISSN
1523-7060

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πŸ“œ SIMILAR VOLUMES

Inside Cover: Manipulating the Expressio
Inside Cover: Manipulating the Expression Rate and Enantioselectivity of an Epoxide Hydrolase by Using Directed Evolution (ChemBioChem 10/2011)
✍ Prof. Dr. Manfred T. Reetz; Dr. Huabao Zheng πŸ“‚ Article πŸ“… 2011 πŸ› John Wiley and Sons 🌐 English βš– 401 KB

The inside cover picture shows the increase in expression efficiency and stereoselectivity of the epoxide hydrolase ANEH induced by directed evolution. Also illustrated is the complementary construct of fused ANEH linker a-fragments in the pMALC4X plasmid used in blue-colony screening. For more deta