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Directed Evolution of an Enantioselective Epoxide Hydrolase: Uncovering the Source of Enantioselectivity at Each Evolutionary Stage

โœ Scribed by Reetz, Manfred T.; Bocola, Marco; Wang, Li-Wen; Sanchis, Joaquin; Cronin, Annette; Arand, Michael; Zou, Jinyu; Archelas, Alain; Bottalla, Anne-Lise; Naworyta, Agata; Mowbray, Sherry L.


Book ID
120346374
Publisher
American Chemical Society
Year
2009
Tongue
English
Weight
582 KB
Volume
131
Category
Article
ISSN
0002-7863

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The inside cover picture shows the increase in expression efficiency and stereoselectivity of the epoxide hydrolase ANEH induced by directed evolution. Also illustrated is the complementary construct of fused ANEH linker a-fragments in the pMALC4X plasmid used in blue-colony screening. For more deta