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Manipulating the Expression Rate and Enantioselectivity of an Epoxide Hydrolase by Using Directed Evolution

โœ Scribed by Prof. Dr. Manfred T. Reetz; Dr. Huabao Zheng


Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
372 KB
Volume
12
Category
Article
ISSN
1439-4227

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Inside Cover: Manipulating the Expressio
โœ Prof. Dr. Manfred T. Reetz; Dr. Huabao Zheng ๐Ÿ“‚ Article ๐Ÿ“… 2011 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 401 KB

The inside cover picture shows the increase in expression efficiency and stereoselectivity of the epoxide hydrolase ANEH induced by directed evolution. Also illustrated is the complementary construct of fused ANEH linker a-fragments in the pMALC4X plasmid used in blue-colony screening. For more deta