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Directed evolution of metagenome-derived epoxide hydrolase for improved enantioselectivity and enantioconvergence

✍ Scribed by Kotik, Michael; Zhao, Wei; Iacazio, Gilles; Archelas, Alain


Book ID
121338881
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
800 KB
Volume
91
Category
Article
ISSN
1381-1177

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The inside cover picture shows the increase in expression efficiency and stereoselectivity of the epoxide hydrolase ANEH induced by directed evolution. Also illustrated is the complementary construct of fused ANEH linker a-fragments in the pMALC4X plasmid used in blue-colony screening. For more deta