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Directed Evolution of Brain-Derived Neurotrophic Factor for Improved Folding and Expression in Saccharomyces cerevisiae

✍ Scribed by Burns, M. L.; Malott, T. M.; Metcalf, K. J.; Hackel, B. J.; Chan, J. R.; Shusta, E. V.


Book ID
125833778
Publisher
American Society for Microbiology
Year
2014
Tongue
English
Weight
751 KB
Volume
80
Category
Article
ISSN
0099-2240

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## Abstract Because of its eukaryotic nature, simple fermentation requirements, and pliable genetics, there have been many attempts at improving recombinant protein production in __Saccharomyces__ __cerevisiae__. These strategies typically involve altering the expression of a native protein thought