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Enhancement of Embryogenesis and Plant Regeneration from Barley Anther Culture by Low Doses of Gamma Irradiation

โœ Scribed by M. I. E. Arabi, B. Al-Safadi, M. Jawhar and N. Mir-Ali


Book ID
124864534
Publisher
Society for In Vitro Biology
Year
2005
Tongue
English
Weight
161 KB
Volume
41
Category
Article
ISSN
1475-2689

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Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of

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Embryogenic cultures and somatic embryos were obtained from immature zygotic embryos of oak (Quercus robur L.) cultured on a modified MS medium and WPM containing BAP (1 mgยทl(-1)) and GA3 (1 mgยทl(-1)) or BAP and IBA. Germination and conversion of oak somatic embryos into plantlets was achieved on WP