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Embryogenesis and plant regeneration from anther cultures of Cucumis sativus L.

✍ Scribed by H.G. Ashok Kumar; H.N. Murthy; K.Y. Paek

Book ID: 117482638
Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 195 KB
Volume: 98
Category: Article
ISSN: 0304-4238
DOI: 10.1016/s0304-4238(03)00003-7

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Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of