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Embryogenesis and plant regeneration from anther culture of bamboo (Sinocalamus latiflora(Munro) McClure)

✍ Scribed by H. S. Tsay; C. C. Yeh; J. Y. Hsu

Publisher
Springer
Year
1990
Tongue
English
Weight
631 KB
Volume
9
Category
Article
ISSN
0721-7714

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✦ Synopsis

Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of response for callus induction. Prolonged culture of these embryogenic calli on the original medium or subculture to an auxin-free medium resulted in embryoid formation and their subsequent germination to form rooted plantlets. Chromosome counts from root-tip cells of anther-derived plant indicated that they were haploid (N=36).

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