✦ LIBER ✦

Embryogenesis and plant regeneration from tissue culture of Canada wildrye,Elymus canadensisL.

✍ Scribed by C. H. Park; P. D. Walton

Publisher: Springer
Year: 1989
Tongue: English
Weight: 573 KB
Volume: 8
Category: Article
ISSN: 0721-7714
DOI: 10.1007/bf00274132

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✦ Synopsis

Somatic embryogenesis and plant regeneration of Canada wildrye (Elymus canadensis L.) from tissue culture was investigated by culturing immature embryos and inflorescences on MS medium containing 2 mg/l 2,4-D. The optimum size of explants for maximum embryogenic callus formation was 1.0 to 1.5 mm for embryos and 4 to 6 cm for inflorescences. Plant regeneration from the subcultured embryogenic callus was attempted monthly using hormone-free MS medium or MS medium with 0.5 mg/1 2,4-D and 0.3 mg/l GA3. Three hundred and fifty seven plantlets were regenerated from the callus cultures of both explant sources during a six month period. Ten chlorophyll deficient plants accounting for 2.8% of the total regenerants were observed. One plant with white striped leaves survived and was found to be an octoploid.

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