Plant regeneration from calli was carried out at two locations using several parental genotypes and environments. Selfed immature diploid (VV) barley (Hordeum vulgare) embryos and immature haploid (V) or hybrid (VB) embryos from crosses between H. vulgare and H. bulbosum were used as explants. 'Gold
Tissue culture and plant regeneration from immature embryo explants of Barley,Hordeum vulgare
โ Scribed by C. S. Goldstein; W. E. Kronstad
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 537 KB
- Volume
- 71
- Category
- Article
- ISSN
- 0040-5752
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Two finely-dispersed, homogeneous and regenerable cell suspension cultures were established from embryogenic callus derived from immature and mature embryos in barley. The quality and viability of suspension cells obtained were determined using differential-interference-contrast microscope and fluor