Enhancement of embryogenesis and plant regeneration from barley anther culture by low doses of gamma irradiation
โ Scribed by M. I. E. Arabi; B. Al-Safadi; M. Jawhar; N. Mir-Ali
- Book ID
- 111732652
- Publisher
- Society for In Vitro Biology
- Year
- 2005
- Tongue
- English
- Weight
- 60 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1475-2689
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Embryogenic callus was initiated from bamboo (Sinocalumus satiflora (Munro) McClure) anthers cultured on N6 medium supplemented with 1 mg/l 2,4-D, 1 mg/l BA, 2 g/l charcoal, 0.8% agar (Sigma) and 9% sucrose. Anthers with microspores at miduninucleate to early-binucleate stages showed better rate of
Embryogenic cultures and somatic embryos were obtained from immature zygotic embryos of oak (Quercus robur L.) cultured on a modified MS medium and WPM containing BAP (1 mgยทl(-1)) and GA3 (1 mgยทl(-1)) or BAP and IBA. Germination and conversion of oak somatic embryos into plantlets was achieved on WP