๐”– Bobbio Scriptorium
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Engineering antibody affinity and specificity

โœ Scribed by D. M. Webster; S. Roberts; J. C. Cheetham; R. Griest; A. R. Rees

Publisher
John Wiley and Sons
Year
1988
Tongue
French
Weight
375 KB
Volume
41
Category
Article
ISSN
0020-7136

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โœฆ Synopsis

A combination of ab initio calculations, "knowledge-based prediction", molecular graphics and site-directed mutagenesis has enabled us to probe the molecular details of antibody:antigen recognition and binding and to alter the affinity and specificity of an antibody for its antigen. The significance of electrostatic hydrogen bonding, hydrophilic/hydrophobic patch matching and van der Waals interactions as well as CDR:CDR interactions are discussed in relation to the results of site-directed mutagenesis experiments on the anti-lysozyme antibody Gloop2. The ability to generate reconstructed antibodies, chimeric antibodies, catalytic antibodies and the use of modelled antibodies for the design of drugs is discussed.

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