Affinity and specificity of penicillin-antibody interaction determined by an enzyme (E. coli β-galactosidase) immunoassay

Requirement of the immunogen (E. coli β-

Requirement of the immunogen (E. coli β-galactosidase) for the response towards a determinant responsible for antibody-mediated enzyme activation, while antibodies binding some other sites can be elicited by mitogen alone

✍ Enza Piccolella; Dora Centis; Franco Celada 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 497 KB

## Abstract Virgin mouse spleen cells cultured __in vitro__ without antigen or mitogen produce a measurable amount of IgM binding __E. coli__ β‐galactosidase (β‐gal). Lipopolysaccharide (LPS) and LPS plus antigen enhance this response, which cannot be considered truly polyclonal since it does not i