The Bayer Immuno 1 TM PSA Assay measures free and ACT-complexed PSA on an equimolar basis, although it uses a monoclonal antibody (MM1) for capture and polyclonal antibodies for detection. Competitive inhibition studies using antibodies directed at various epitopes on PSA and PSA-ACT demonstrated th
Microscale characterization of the binding specificity and affinity of a monoclonal antisulfotyrosyl IgG antibody
β Scribed by Klaus S. Lassen; Andrew R. M. Bradbury; Jens F. Rehfeld; Niels H. H. Heegaard
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 458 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
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Understanding the structural and dynamic determinants of binding free energy in the antigen-antibody bond is of great interest. Much work has focused on selective mutations in order to locate key interaction residues, but this generally results in reduced affinity. The present work instead examines