A homogeneous enzyme immunoassay has been developed in which an antigen and glucose-6-phosphate dehydrogenase are coimmobilized on agarose beads. Binding of hexokinase-labeled antibody to the bead-bound antigen results in an accelerated conversion of glucose, ATP, and NAD+ to 6-phosphogluconolactone
Engineering a regulatable enzyme for homogeneous immunoassays
β Scribed by Fastrez, Jacques; Legendre, Daniel; Soumillion, Patrice
- Book ID
- 109826610
- Publisher
- Nature Publishing Group
- Year
- 1999
- Tongue
- English
- Weight
- 545 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/5243
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