Electrostatic and electrokinetic potentials in two polymer aqueous phase systems
β Scribed by Donald E Brooks; Kim A Sharp; Stephan Bamberger; Cherry H Tamblyn; Geoffrey V.F Seaman; Harry Walter
- Publisher
- Elsevier Science
- Year
- 1984
- Tongue
- English
- Weight
- 946 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0021-9797
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π SIMILAR VOLUMES
Two-polymer aqueous phase systems are described in which ribosomes selectively partition into one of the phases. One of the phase systems is used to determine rapidly and conveniently binding of thiostrepton and erythromycin to Escherichia co/i ribosomes under equilibrium conditions.
## Abstract A proteolytic enzyme, thermolysin, was covalently immobilized to dextran which has a small number of carboxyl groups. Enzymatic synthesis of aspartame derivative (BocβAspβPheβOMe) using the dextranβconjugated thermolysin was carried out in an aqueous polymer twoβphase system, where poly