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Electrospray mass spectrometry of the glycosylinositol phospholipid of the scrapie prion protein

✍ Scribed by STAHL, N; BALDWIN, M; PRUSINER, S


Book ID
123238617
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
692 KB
Volume
15
Category
Article
ISSN
0309-1651

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Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces spe