Permethylation and tandem mass spectrome
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M.A. Baldwin; N. Stahl; L.G. Reinders; B.W. Gibson; S.B. Prusiner; A.L. Burlinga
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Article
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1990
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Elsevier Science
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English
β 761 KB
Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces spe