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Identification of glycoinositol phospholipid-linked and truncated forms of the scrapie prion protein

โœ Scribed by Stahl, Neil; Baldwin, Michael A.; Burlingame, Alma L.; Prusiner, Stanley B.

Book ID
127109666
Publisher
American Chemical Society
Year
1990
Tongue
English
Weight
709 KB
Volume
29
Category
Article
ISSN
0006-2960

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Permethylation and tandem mass spectrome
Permethylation and tandem mass spectrometry of oligosaccharides having free hexosamine: Analysis of the glycoinositol phospholipid anchor glycan from the scrapie prion protein
โœ M.A. Baldwin; N. Stahl; L.G. Reinders; B.W. Gibson; S.B. Prusiner; A.L. Burlinga ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 761 KB

Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces spe