✦ LIBER ✦

Permethylation and tandem mass spectrometry of oligosaccharides having free hexosamine: Analysis of the glycoinositol phospholipid anchor glycan from the scrapie prion protein

✍ Scribed by M.A. Baldwin; N. Stahl; L.G. Reinders; B.W. Gibson; S.B. Prusiner; A.L. Burlingame

Book ID: 102985111
Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 761 KB
Volume: 191
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90405-x

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✦ Synopsis

Permethylation of the glycan isolated from the glycoinositol phospholipid (GPI) anchor of the scrapie prion protein (PrPSc) trimethylates a free hexosamine to form a quarternary ammonium salt, substantially increasing the sensitivity for analysis by mass spectrometry. This derivatization induces specific fragmentation reactions in collision-induced dissociation spectra obtained on a four-sector tandem mass spectrometer, identifying the branching pattern of the PrPSc GPI glycan.