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Electrophoretic transfer technique to detect and identify amylases in polyacrylamide gels

✍ Scribed by V. H. Mulimani; S. Thippeswamy


Book ID
109155180
Publisher
The American Society for Biochemistry and Molecular Biology
Year
2001
Tongue
English
Weight
372 KB
Volume
29
Category
Article
ISSN
1470-8175

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A method for the analysis of multiple forms of glucan-degrading enzymes is described. The procedure consists of the separation of the proteins by electrophoresis or isoelectric focusing in glucan-free polyacrylamide gels followed by the nondenaturing electrophoretic transfer into a second polyacryla