Multiple forms of amylase in leaf extracts: Electrophoretic transfer of the enzyme forms into amylose-containing polyacrylamide gels
✍ Scribed by Martin Steup; Klaus-Peter Gerbling
- Book ID
- 102984781
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 659 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A method for the analysis of multiple forms of glucan-degrading enzymes is described. The procedure consists of the separation of the proteins by electrophoresis or isoelectric focusing in glucan-free polyacrylamide gels followed by the nondenaturing electrophoretic transfer into a second polyacrylamide layer which contains immobilized glucans. The method combines the resolving power of electrophoretic separations in glucan-free media with the sensitivity of amylase activity detection in amylose-containing polyacrylamide gels. The procedure is especially useful when samples containing low amylase activity, but a large number of multiple enzyme forms, are to be analyzed.