From gels to chips: “Minisequencing” pri
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Ann-Christine Syvänen
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Article
📅
1999
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John Wiley and Sons
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English
⚖ 383 KB
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In the minisequencing primer extension reaction, a DNA polymerase is used specifically to extend a primer that anneals immediately adjacent to the nucleotide position to be analyzed with a single labeled nucleoside triphospate complementary to the nucleotide at the variant site. The reaction allows