𝔖 Bobbio Scriptorium
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Melting gel techniques in single nucleotide polymorphism and mutation detection: From theory to automation

✍ Scribed by Jens Bjørheim; Gustav Gaudernack; Per Olaf Ekstrøm


Book ID
101393950
Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
347 KB
Volume
25
Category
Article
ISSN
1615-9306

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In the minisequencing primer extension reaction, a DNA polymerase is used specifically to extend a primer that anneals immediately adjacent to the nucleotide position to be analyzed with a single labeled nucleoside triphospate complementary to the nucleotide at the variant site. The reaction allows