Electrochemical detection of reducing carbohydrates in high-performance liquid chromatography after post-column derivatization with 2-cyanoacetamide

✍ Scribed by Susumu Honda; Tadao Konishi; Shigeo Suzuki

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 461 KB
• Volume: 299
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)97836-7

✦ Synopsis

Reducing carbohydrates were derivatized post-column with 2-cyanoacetamide to readily oxidizable compounds. Electrochemical detection allowed sensitive and reproducible monitoring of glucose with a detection limit and a linear range of 20 pmol and 50 pmol-2 nmol, respectively. Similar results were obtained with other reducing carbohydrates. This method was universally applicable to high-performance liquid chromatography in gel-permeation, ion-exchange and partition modes.