Reducing carbohydrates were derivatized post-column with 2-cyanoacetamide to readily oxidizable compounds. Electrochemical detection allowed sensitive and reproducible monitoring of glucose with a detection limit and a linear range of 20 pmol and 50 pmol-2 nmol, respectively. Similar results were ob
β¦ LIBER β¦
Determination of Ubiquinone in Blood by High-Performance Liquid Chromatography with Post-Column Fluorescence Derivatization Using 2-Cyanoacetamide
β Scribed by Yukio Nohara; Junko Suzuki; Hiroaki Kubo
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 323 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1053-0509
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An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r > 0.99