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Post-column derivatization of carbohydrates with ethanolamine—boric acid prior to their detection by high-performance liquid chromatography

✍ Scribed by M.J. Del Nozal; J.L. Bernal; F.J. Gomez; A. Antolin; L. Toribio


Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
566 KB
Volume
607
Category
Article
ISSN
1873-3778

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## Abstract A rapid and highly sensitive determination method for urinary free cortisol has been developed using reversed phase high performance liquid chromatography (HPLC) with a precolumn for sulphuric acid‐ethanol fluorescence derivatization and column switching. Urinary cortisol, eluted from t