Efficient High-Performance Liquid Chromatography with Liquid-Liquid Partition Cleanup Method for the Determination of Pymetrozine in Tobacco

✍ Scribed by Xian Zhang; Xinsheng Cheng; Chuanqin Wang; Zheng Xi; Qiuhua Li

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 158 KB
• Volume: 97
• Category: Article
• ISSN: 0003-4592
• DOI: 10.1002/adic.200790015

✦ Synopsis

Abstract

For the first time, a novel, simple and reliable method for analysis of pymetrozine residues in flue‐cured tobacco leaves has been developed utilizing HPLC‐UV with liquid‐liquid partition cleanup. Pre‐treatment with ultrasonic extraction and liquid‐liquid partition procedures gave preferable baseline separation and clean chromatograms by removing water‐soluble and fat‐soluble components which interfere with pymetrozine in the test. The performance of the method was evaluated and validated: the detection limit (LOD) was 0.005μg.mL^‐1^, the relative standard deviation (RSD) was 1.2 % (n=5), and the overall recovery was above 90 % at fortification levels of 0.200, 0.500, 1.000, and 5.000 mg.kg^‐1^. The proposed method was successfully employed for the determination of pymetrozine residues in twelve flue‐cured tobacco samples collected from different regions of China.