Determination of saccharides in sake by high-performance liquid chromatography with polarized photometric detection

✍ Scribed by Kazuichi Hayakawa; Kyoko Ando; Natsue Yoshida; Atsushi Yamamoto; Akinobu Matsunaga; Masayuki Nishimura; Mitsuo Kitaoka; Keizo Matsui

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 68 KB
• Volume: 14
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(200004)14:2<72::aid-bmc927>3.0.co;2-s

✦ Synopsis

A high-performance liquid chromatographic method has been developed for the determination of saccharides in sake, an alcoholic beverage brewed from rice. Saccharides in sake were separated on a normal phase (carbamoyl bonded silica) column using a linear gradient elution of water in acetonitrile. Seven saccharides, glucose, maltose, isomaltose, maltotriose, panose, isomaltotriose and ethyl a-D-glucoside, were determined by a polarized photometric detector. Unidentified peaks suggesting saccharides with polymerization degrees over 4 were also observed. The proposed method did not require any sample clean-up treatment. As an application, saccharide compositions in various kinds of sake were compared.