Determination of morphine 3-esters in rabbit plasma by high-performance liquid chromatography with ultraviolet detection

✍ Scribed by Karin Otter; Christian Mignat; Dieter Heber; Albrecht Ziegler

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 67 KB
• Volume: 14
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/1099-0801(200008)14:5<327::aid-bmc991>3.0.co;2-g

✦ Synopsis

A sensitive high-performance liquid chromatographic (HPLC) method for the quantitation of the morphine 3-esters 1[3-(2,2-dimethylvaleroyl)-morphine (A), 3-(2-phenylbenzoyl)-morphine (B) and 3-(2,2-diphenylpropionyl)-morphine (C)] in rabbit plasma is described. Sample preparation was based on reversed-phase solid-phase extraction. The compounds were separated on C 18 reversed-phase analytical columns and then determined by ultraviolet detection. The recovery from plasma was 78.7 AE 7.4%, 69.1 AE 6.9% and 75 AE 7.2% (mean AE SD) for A, B, and C, respectively. The present method enabled the detection limit of 0.2, 0.2 and 0.1 ng and quantification limit of 20, 10 and 10 ng/ml for A, B and C, respectively. The developed method was used for determination of the plasmakinetics of these morphine 3-esters in rabbits.