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Determination of ginsenosides in medicinal plants and health supplements by pressurized liquid extraction (PLE) with reversed phase high performance liquid chromatography

✍ Scribed by Hwei Khien Lee; Hwee Ling Koh; Eng Shi Ong; Soo On Woo

Publisher: John Wiley and Sons
Year: 2002
Tongue: English
Weight: 324 KB
Volume: 25
Category: Article
ISSN: 1615-9306
DOI: 10.1002/1615-9314(20020201)25:3<160::aid-jssc160>3.0.co;2-#

No coin nor oath required. For personal study only.

✦ Synopsis

Determination of ginsenosides in medicinal plants and health supplements by pressurized liquid extraction (PLE) with reversed phase high performance liquid chromatography

A method using dynamic pressurized liquid extraction (PLE) was developed to extract ginsenosides in Panax ginseng, American ginseng, and health supplement products with methanol. The extraction efficiency of PLE was comparable to that obtained by Soxhlet extraction. The method precision (RSD) was found to vary from 1.5 to 13.0% for the various ginsenosides, higher RSD values were observed for lower level ginsenosides present in the medicinal plants or health supplement products. Separation of ginsenosides in the methanolic extracts was achieved with a water -acetonitrile gradient system using a C-18 reversed phase column.

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