✦ LIBER ✦

Quantitative determination of maitenin and 22β-hydroxymaitenin in callus of Maytenus aquifolium (celastraceae) by reverse phase high performance liquid chromatography

✍ Scribed by Joaquim Corsino; Alberto Camilo Alécio; Maria Lúcia Ribeiro; Maysa Furlan; Ana Maria S. Pereira; Ines Batista Duarte; Suzelei Castro França

Publisher: John Wiley and Sons
Year: 1998
Tongue: English
Weight: 73 KB
Volume: 9
Category: Article
ISSN: 0958-0344
DOI: 10.1002/(sici)1099-1565(199809/10)9:5<245::aid-pca413>3.0.co;2-t

No coin nor oath required. For personal study only.

✦ Synopsis

Explants of Maytenus aquifolium were induced to form callus and, subsequently, suspension cultures. The isolation of natural products from callus led to the identification of the cytotoxic triterpene quinonemethides, maitenin (1) and 22b-hydroxymaitenin (2). A rapid, sensitive and reliable reversedphase high-performance liquid chromatography method was developed using a C 18 column and isocratic elution for the determination of 1 and 2. The elaborated method gave well-resolved peaks for these compounds with good detection response and linearity in the range of 0.08-72.0 mg. The quantification of 1 and 2 was performed by an external standard method.

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