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Effect of Three Elution Buffers on the Recovery and Structure of Monoclonal Antibodies

โœ Scribed by Linda O. Narhi; D.J. Caughey; Thomas Horan; Yoshiko Kita; David Chang; Tsutomu Arakawa

Book ID
102559821
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
271 KB
Volume
253
Category
Article
ISSN
0003-2697

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## Abstract Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pHโ€based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evalua