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pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies

✍ Scribed by Paul K. Ng; Mark A. Snyder

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
293 KB
Volume
35
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH‐based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuviaβ„’ S), which has high binding capacity. A three‐column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50‐cycle study.

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