Impact of freezing on pH of buffered solutions and consequences for monoclonal antibody aggregation
β Scribed by Parag Kolhe; Elizabeth Amend; Satish K. Singh
- Book ID
- 102774358
- Publisher
- American Institute of Chemical Engineers
- Year
- 2009
- Tongue
- English
- Weight
- 732 KB
- Volume
- 26
- Category
- Article
- ISSN
- 8756-7938
- DOI
- 10.1002/btpr.377
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Freeze-thawing is a potentially damaging stress to which therapeutic proteins can be exposed deliberately during storage of bulk drug substance, and accidentally because of mishandling of commercial product during shipping and/or storage. The primary route of degradation induced by freeze-thawing is
Changes in protein-protein interactions, protein unfolding, and nonnative aggregation were assessed for a series of human IgG1 antibodies as a function of pH and solution ionic strength (I). Unfolding transitions were characterized with differential scanning calorimetry. Protein-protein interactions