Effect of substrate decomposition on the spectrophotometric assay of dihydrofolate reductase
โ Scribed by B.L. Hillcoat; P.F. Nixon; R.L. Blakley
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 776 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0003-2697
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๐ SIMILAR VOLUMES
A new spectrophotometric method is developed and applied for the study of the inhibitory effect of triamterene, hydrochlorothiazide and their combinations on the in vitro activity of dihydrofolate reductase enzyme. The method is based on incubating the drug (0.1-1.0 microM) or a buffer control with
FoIate-Seph~ose at&ity column material has been prepared and used for the isolation of dihydrofolate reductases from three sources: (a) ~ethopte~n-resist~t Ll210 cells in culture; (b) amethopterin-resistant LactobaciHus casei; and (c) r To whom requests for reprints should be addressed.