Preparation of tritiated dihydrofolate and its use in the assay of dihydrofolate reductase
โ Scribed by Sigmund F. Zakrzewski; Jaakko J. Himberg
- Publisher
- Elsevier Science
- Year
- 1971
- Tongue
- English
- Weight
- 536 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The ability of the enzyme dihydrofolate reductase to catalyze the formation of tetrahydrobiopterin from dihydrobiopterin was used to develop a method for measuring the activity ofthis enzyme in viva. This method can be used to determine the activity of the enzyme in tissues as well as the extent and
We developed a method to determine dihydrofolate reductase (DHFR) activity at pH 7.4 (37ยฐC) by monitoring its product, tetrahydrofolate (H 4 folate), using HPLC with electrochemical detection. After the assay mixture was deproteinized by 0.5 M perchlolic acid, the H 4 folate concentration was measur
We have previously demonstrated systemic resistance to methotrexate (MTX) in transgenic mice carrying a foreign, mutant dihydrofolate reductase (DHFR, E.C. 1 S.1.3) gene. The new gene was introduced as a cDNA cloned into an expression vector driven by the simian virus 40 (SV40) early promoter. Previ